日粮纳米锌水平对公羊睾丸和附睾Cu-ZnSOD表达的影响

doi:10.11843/j.issn.0366-6964.2014.10.007

Abstract

Abstract:

The objectives of this study were to evaluate the expression of copper zinc superoxide dismutase and its cellular localization in testis and epididymis of ram lambs from different levels of Nano-zinc supplementation groups.Sixteen 9-month-old Jinzhong ram lambs with approximately weight and good healthy were randomly divided into 4 treatments，fed with a basal diet with supplementation of 0，50，100 and 150 mg•kg^-1 DM Nano-zinc respectively.Experimental period was 90 d.At the end of test，the samples of testis and epididymis were collected.Expression of Cu-ZnSOD mRNA and protein was detected by real-time fluorescent quantitative PCR and Western blotting.Cellular localization of Cu-ZnSOD in testis was examined by immunohistochemistry.The quantitative real-time PCR results showed the rank order of Cu-ZnSOD mRNA expression was caput ≥testis > corpora >cauda.The mRNA and protein expression of Cu-ZnSOD in testis and epididymis were higher in the group with supplementation of 50 and 100 mg•kg^-1 Nano-zinc than those in control group（P<0.05）.The strong positive signals of Cu-ZnSOD protein were detected in spermatogenic cells of testis，and weak signals in interstitial tissue.The expression of Cu-ZnSOD was different among testis，caput，corpora and cauda of epididymidis.The optimal supplementation of Nano-zinc in diet could improve the expression of Cu-ZnSOD in testis and epididymis of rams.And the mechanisms needs to be further researched.

CLC Number:

S826.2

REN You-she，QIN Xiao-wei，GUO Li-na，ZHAO Hui，LIU Wen-zhong，ZHANG Jian-xin,ZHANG Chun-xiang. Effect of the Supplementation of Different Nano-zinc Levels on Expression of Copper Zinc Superoxide in Testis and Epididymis of Ram Lambs[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(10): 1622-1630.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/10.11843/j.issn.0366-6964.2014.10.007

https://www.xmsyxb.com/EN/Y2014/V45/I10/1622

References

［1］BEDWAL R S，NAIR N，MATHUR R S.Effects of zinc deficiency and toxicity on reproductive organs，pregnancy and lactation-a review［J］.Trace Elem Med，1991，8:89-100.
［2］BEDWAL R S，BAHUGUNA A.Zinc，copper and selenium in reproduction［J］.Experientia，1994，50:626-640.
［3］AULD D S.The ins and outs of biological zinc sites［J］. Biometals，2009，22:141-148.
［4］KUMARI D，NAIR N，BEWAL R S.Effect of dietary zinc deficiency on testes of Wistar rats：Morphometric and cell quantification studies［J］.J Trace Elem Med Biol，2011，25(1):47-53.
［5］KUMARI D，NAIR N，BEWAL R S.Testicular apoptosis after dietary zinc deficiency：ultrastructural and TUNEL studies［J］.Syst Biol Reprod Med，2011,57(5):233-243.
［6］WALLACE E，CALVIN H I，SALGO M P，et al.Normal levels of zinc and sulphydryls in morphologically abnormal populations of spermatozoa from moderately zinc deficient rats［J］.Gamete Res，1984，9:375-386.
［7］MERRELLS K J，BLEWETT H，JAMIESON J A，et al.Relationship between abnormal sperm morphology induced by dietary zinc deficiency and lipid composition in testes of growing rats［J］.Br J Nutr， 2009,102(2):226-232.
［8］WANG C L,WU C J，WANG Y R，et al.Study progress of Cu/Zn superoxide disnmtase–from gene to function ［J］.Progr Modern Biomed，2008，8(5)：940-943.
［9］AlVAREZ J G，STOREY B T.Evidence for increased lipid peroxidative damage and loss of superoxide dismutase activity as a model of sublethal cryodamage to human sperm during cryopreservation［J］.J Androl，1992，13:232-242.
［10］SANOCKA D，MIESEL R，JEDRZEJCZAK P，et al.Effect of reactive oxygen species and the activity of antioxidant systems in human semen；association with male infertility［J］.Intern J Andro，1997，20:255-264.
［11］PEEKER R，ABRAMSSON L，MARKLUND S L.Superoxide dismutase isoenzymes in human seminal plasma and spermatozoa［J］. Mol Hum Reprod，1997,3(12):1061-1066.
［12］MRUK D D，SILVESTRINI B，MO M Y，et al.Antioxidant superoxide dismutase–a review：its function，regulation in the testis，and role in male fertility［J］.Contraception，2002,65:305-311.
［13］MURAWSKI M，SACZKO J，MARCINKOWSKA A，et al.Evaluation of superoxide dismutase activity and its impact on semen quality parameters of infertile men［J］.Folia Histochem Cytobiol，2007，45 （Suppl 1）:S123-126.
［14］WAHEED M M，GOUDA E M，KHALIFA T A.Impact of seminal plasma superoxide dismutase and glutathione peroxidase on cryopreserved buffalo spermatozoa［J］.Anim Reprod Sci，2013,142(3-4):126-130.
［15］SØRENSEN M B，STOLTENBERG M，HENRIKSEN K，et al.Histochemical tracing of zinc ions in the rat testis［J］.Mol Hum Reprod，1998,4:423-428.
［16］STOLTENBERG M，SØRENSEN M B，DANSCHER G，et al.Autometallographic demonstration of zinc ions in rat sperm cells［J］.Mol Hum Reprod，1997，3(9):763-767.
［17］FUJIMORI O，TSUKISE A，YAMADA K.Histochemical demonstration of zinc in rat epididymis using a sulphide-silver method［J］.Histochemistry，1988，88(3-6):469-473.
［18］STOLTENBERG M，ERNST E，ANDREASEN A，et al.Histochemical localization of zinc ions in the epididymis of the rat［J］.Histochem J，1996，28(3):173-185.
［19］STOLTENBERG M,LUND L,JUHL S,et al.Histochemical demonstration of zinc ions in human epididymis using autometallography［J］.Histochem J，1997，29(10):721-726.
［20］ROWE M P，POWELL J G，KEGLEY E B，et al.Effect of supplemental trace-mineral source on bull semen quality［J］. Prof Anim Sci，2014,30，1:68-73.
［21］KUMAR N，VERMA R P，SINGH L P，et al.Effect of different levels and sources of zinc supplementation on quantitative and qualitative semen attributes and serum testosterone level in crossbred cattle (Bos indicus ×Bos taurus) bulls［J］.Reprod Nutr Dev，2006，46(6):663-675.
［22］KENDALL N R，MCMULLEN S，GREEN A，et al.The effect of a zinc，cobalt and selenium soluble glass bolus on trace element status and semen quality of ram lambs［J］.Anim Reprod Sci，2000,62(4):277-283.
［23］SAID T M，AGARWAL A，SHARMA R K，et al.Human sperm superoxide anion generation and correlation with semen quality in patients with male infertility［J］.Fertil Steril，2004，82:871-877.
［24］MARZEC-WRÓBLEWSKA U，KAMI?SKI P，LAKOTA P，et al.Zinc and iron concentration and SOD activity in human semen and seminal plasma［J］.Biol Trace Elem Res，2011,143(1):167-177.
［25］KASIMANICKAM R，PELZER K D，KASIMANICKAM V，et al.Association of classical semen parameters，sperm DNA fragmentation index，lipid peroxidation and antioxidant enzymatic activity of semen in ram-lambs［J］.Theriogenology，2006，65(7):1407-1421.
［26］KASIMANICKAM R，KASIMANICKAM V，THATCHER C D，et al.Relationships among lipid peroxidation，glutathione peroxidase，superoxide dismutase，sperm parameters，and competitive index in dairy bulls［J］.Theriogenology，2007，67(5):1004-1012.
［27］CASSANI P，BECONI M T，O'FLAHERTY C.Relationship between total superoxide dismutase activity with lipid peroxidation，dynamics and morphological parameters in canine semen［J］.Anim Reprod Sci，2005，86(1-2):163-173.
［28］ROCA J，RODRIGUEZ M J，GIL M A，et al.Survival and in vitro fertility of boar spermatozoa frozen in the presence of superoxide dismutase and/or catalase［J］. J Andro，2005，26:15-24.
［29］GARRATT M，BATHGATE R，GRAAF S P.Copper-zinc superoxide dismutase deficiency impairs sperm motility and in vivo fertility［J］.Reproduction，2013，146(4):297-304.
［30］TSUNODA S，KAWANO N，MIYADO K，et al.Impaired fertilizing ability of superoxide dismutase 1-deficient mouse sperm during in vitro fertilization［J］.Biol Reprod，2012，87(5):121.
［31］KOBAYASHI T，MIYAZAKI T，NATORI M，et al.Protective role of superoxide dismutase in human sperm motility：superoxide dismutase activity and lipid peroxide in human seminal plasma and spermatozoa［J］.Hum Reprod，1991，6(7):987-991.
［32］CELINO F T，YAMAGUCHI S，MIURA C，et al.Tolerance of spermatogonia to oxidative stress is due to high levels of Zn and Cu/Zn superoxide dismutase［J］.PLoS ONE，2011，6(2):e16938.
［33］BAUMBER J,BALL B A.Determination of glutathione peroxidase and superoxide dismutase-like activities in equine spermatozoa，seminal plasma，and reproductive tissues［J］.Am J Vet Res，2005，66(8):1415-1419.
［34］KOZIOROWSKA-GILUN M，GILUN P，FRASER L，et al.Antioxidant enzyme activity and mRNA expression in reproductive tract of adult male European Bison (Bison bonasus，Linnaeus 1758) ［J］.Reprod Domest Anim，2013，48(1):7-14.
［35］NONOGAKI T，NODA Y，NARIMOTO K，et.al.Localization of CuZn-superoxide dismutase in the human male genital organs ［J］.Hum Reprod，1992，7(1):81-85.
［36］JOW W W，SCHLEGEL P N，CICHON Z，et al.Identification and localization of copper–zinc superoxide dismutase gene expression in rat testicular development［J］.Andrology，1993，14：439-447.

[1]	WANG Haibo, ZHAN Jinshun, GU Zhiyong, CHEN Xinfeng, PAN Yue, JIA Haobin, ZHONG Xiaojun, LI Kairong, ZHAO Shengguo, HUO Junhong. Comparative Study on Meat Quality Characteristics of Three-Way Hybrid Sheep Charolais×Duper×Hu and Charolais×Australian White×Hu and Hu Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 110-119.
[2]	HAN Haoyuan, LI Shikai, YANG Ruiqiao, LI Manman, LI Jun, HA Si, ZHAO Jinyan, WEI Hongfang, QUAN Kai. Mining Key Candidate Genes for High Reproduction Performance of Huai Goats Based on Transcriptome Sequencing [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 5077-5090.
[3]	LI Dengpan, MA Keyan, HAN Jintao, BAI Yaqin, LI Taotao, MA Youji. Detection and Analysis of Whole Genome Selection Signal of Yongdeng Qishan Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4577-4588.
[4]	ZHAO Xueyang, LI Danni, WANG Yuchen, GUO Lei, WANG Li, HUANG Jie, JIAO Yiqiang, AN Xiaopeng, ZHANG Xiyun, ZHANG Lei, SONG Yuxuan. GWAS Analysis of Lambing Traits in East Friesian and Hu Crossbred Sheep and Verification of Candidate Gene GRID2 [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4625-4635.
[5]	ZHANG Hanyue, ZHAO Dan, LIANG Yu, ZHAO Bishi, FAN Mengdan, QIAO Liying, LIU Jianhua, YANG Kaijie, PAN Yangyang, LIU Wenzhong. miR-150 Regulates Ovine Preadipocyte Differentiation by Targeting AOC3 [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3262-3274.
[6]	LIU Linli, PENG Xuelan, LI Bo, CHENG Lefan, CIREN Lamu, ZHANG Enping. Effect of Overexpression of UCP3 Gene on the Differentiation of Sahu Sheep Preadipocytes [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3275-3285.
[7]	WANG Wannian, CHEN Sijia, GAO Jinrong, WEN Zhonghao, YUAN Mengjiao, ZHANG Hongzhi, PANG Zhixu, QIAO Liying, LIU Wenzhong. Simulation Study on Genomic Selection of Sex-limited Traits Using Multilayer Perceptron in Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2824-2835.
[8]	DONG Yajie, HAO Xiaojing, WU Jinqiang, WANG Rong, ZHANG Pengxiang, WANG Haidong, HE Xiaoyan. Exploration of the Effect of SHH on Wool Bending through Krox20 Regulation of IGFBP5 Expression Based on Sheep Keratinocytes [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2365-2375.
[9]	BI Yazhen, SHANG Mingyu, HU Wenping, ZHANG Li. Correlation and Regression Analysis among Growth Traits and Association Analysis of TRHDE Gene Polymorphism with Growth Traits in Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1415-1428.
[10]	LI Yinxia, YA Shengjiang·Nasier, SAI Like·Duman, QIAN Yong, CAO Shaoxian, WANG Weilie, MENG Chunhua, ZHANG Jun, ZHANG Jianli. Evaluation of Genetic Diversity and Genetic Structure in Kirgiz Sheep Population Based on SNPs Chip [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(2): 572-583.
[11]	LI Ling, LI Yefang, LIANG Benmeng, SUN Yujiang, MA Yuehui, MA Qing, JIANG Lin, LIU Shuqin. Paternity Identification of Tan Sheep Based on SNP Markers [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(9): 2912-2919.
[12]	ZHOU Shiwei, WU Tingjie, WANG Qian, CAI Bei, HAN Saizheng, WU Yanhu, WANG Xiaolong, CHEN Yulin. Establishment of the Efficient Method to Detect the FecB Mutation and the Construction of the Prolific Tan Sheep Population [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(9): 2920-2929.
[13]	DING Ziqiang, GE Wenbo, DUAN Hongwei, LÜ Jianshu, ZENG Jianlin, WANG Wenjuan, NIU Tian, ZHANG Yong, ZHAO Xingxu, HU Junjie. Dihydrotestosterone Affects Sheep Uterine Function through Regulating Progesterone, Oestrogen and Apoptosis [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(9): 2993-3005.
[14]	LI Tingting, LIU Qiuyue, LI Xiangchen, WANG Haitao. Research Progress and Applications of Genes Associated with Economic Traits in Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(8): 2417-2434.
[15]	XU Songwei, XIANG Hua, ZHANG Huanrong, YANG Lu, WANG Xianjun, SALANG Wenzhu, ZHU Jiangjiang. Effects of Interfering SERBP1 on the Cell Proliferation, Cell Cycle and Apoptosis in Goat Turbinate Bone Primary Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(8): 2708-2720.

Effect of the Supplementation of Different Nano-zinc Levels on Expression of Copper Zinc Superoxide in Testis and Epididymis of Ram Lambs

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments